[Toxicity Evaluation of coral madrepora and human BMG in peripheral mononuclear blood cells]

Disadvantage of auto grafts make widespread use of other bone graft materials such as xenografts and allografts. cytotoxic effect of materials and biomaterials on Lively human cells is the Object that always should be observed. In present study had tried to comparison evaluation cytotoxic effete of two bio material -human BMG and coral madrepora -on human peripheral mono nuclear blood cells. In present experimental study after to make preparation peripheral blood with fycole method and After preparation and sterilization of human BMG and coral madrepora, Then, human BMG and coral madrepora, aside to gether are divided into 10, 20, 50 mg portions and cultured on 24-well of 100000 mononuclear cells of peripheral blood and they are evaluated after 24, 48 and 72 hours. MTT method was used for evaluation of cytotoxic effect using ELISA reader in 540 nm wave lengths. Light absorption rate depends on MTT uptake by the cells, indicating vital activity of the cells. Result evaluated with variance and whilcoxon-rank sum and mann u whitnney test. All of the human BMG doses that were used weren,t toxic and all of them increased number of the human peripheral blood mononuclear cells [P< 0.005]. But coral madrepora, was decried number of the human peripheral blood mononuclear cells after 24 hour in 50 mg doses and after 48 hours in 10 mg doses and after 72 hours in 20 and 50 mg doses. With increasing of human BMG concentration and passing time, increasing of human peripheral blood mononuclear cells can be observed and it has a statistically significant difference with control group. Therefore, as a conclusion, human BMG is compatible with human blood mononuclear cells. But coral madrepora had toxicity for this blood cells. However for more judgment, need more study with different cells

Multi vesicular osseous hydatid disease of the mandible- a case report

Hydatid disease is a common and major public health issue caused by parasite Echinococcus granulosus. The highest prevalence of the parasite can be found in different parts of world like Africa, Australia, and South America. This infection can occurs in almost any part of the body. Here we present clinical, radiological, histological features and treatment of a multi vesicular osseous hydatid disease of the mandible in an Afghan 5 year old boy with a firm swelling in the right side of mandible

[Eighteen years follow up of a patient with Gorlin syndrome; case report]

The nevoid basal cell carcinoma syndrome is a rare and autosomal dominant inherited disease with well-defined characteristics, summarized by Gorlin and Goltz in 1960. In the head and neck region, cerebral calcifications, Basal Cell Carcinoma [BCC] and multiple Odontogenic Kerato Cysts [OKC] of the jaws are the predominant findings. Odontogenic keratocysts with high rate of recurrency and basal cell carcinoma of the facial skin are important to dentists. In this article we present a 23 year old male with multiple recurrent cysts of the jaws who diagnosed to have Gorlin syndrome. The patient also has polydactily, palmar and plantar pits, bifid ribs, hypertelorism, calcification of falx cerebri and missing of four wisdom teeth. Diagnosis, treatment and follow up of this case are discussed

[Evaluation of suture removal time and wound healing after third molar surgery]

Suture removal time is one of the most controversial issues after third molar surgery. The purpose of this study was to evaluate wound healings in relation with suture removal time after 5, 8, and 10 days following the third molar surgery. This clinical trial study with double blind method was conducted on 90 patients in need of third molar surgery. Patients were non-smokers and had no history of known systemic diseases. Sex and age range were similar in all study groups. The same suturing material as well as post surgery medications were used for all participants. Patients were then divided into 3 groups in order to have their suture removal at 5[th] [group A], 8[th] [group B], and 10[th] [group C] day following the surgery. Statistical analysis was conducted and Kruskal-Wallis and chi [2] tests were used for analyzing group differences and significance level by evaluating wound tension, wound healing and inflammation status. Group A, was almost without wound tension or had just a negligible amount of tension. Group B had 33% and group C had 17% of this condition [P<0.01]. Moderate to severe inflammation was measured 70% in group A, 23.3% in group B and 20% in group C [P<0.001]. Wound healing was 83% in group A, 17% in group B and 10% in group C [P<0.001] .Amount of tension in suture was 13.3% in group A, 70% in group B and 86.7% in group C [P<0.001]. Based on the results of this study, it seems that time factor plays an important role in wound healing. This is true as long as the suture tension is not reduced. Therefore, 8 days after the surgery is the best time for sutures removal

[Histological evaluation of the effects of human bone matrix gelatin [BMG] with autogenous bone graft in the reconstruction of parietal bone defect in rats]

Autogenous bone grafts are commonly used for the reconstruction of bone defects in surgical procedures. The complication of preparing bone grafts and their application are important factors that have encouraged investigators to use human bone matrix gelatin human [BMG] which is one of the most popular graft materials. Because of the worldwide need for reconstruction of bone defects and its difficulty the aim of this study was to evaluate and compare the effects of BMG and auto graft on the reconstruction of bone defects in parietal bone of rats. In this experimental study, data was collected through microscopic observations. Human BMG produced using human Humorous bone based on Urist technical method. 12 healthy male Sprague dawley rats randomly assigned into 4 groups [3 rats in each group] and sacrificed in 7, 14, 24 and 60[th] days after surgery. Two defects were made in the left and right sides of parietal bone of all rats. Human BMG placed randomly in one side and auto graft in the other side and then all specimens in each group were assessed and compared histologically to each other. Assessments included amount, rate and type of bone formation, inflammation signs, fibrous tissue and cartilage formation to analyze the histologic findings, Wilcoxon-single rank test was used. The results showed a reduction of inflammation and also increase in new bone formation in both groups during 7, 14, 24 and 60th day after surgery. Bone formation percentage in human BMG cases in 24th day was more than autograft. There was no significant difference between two groups in 60th day. The findings suggested that although human BMG has the same effects as autograft, the rate of reconstruction with human BMG is more